Comments on IVF Authority: CGH Egg/ Embryo Selection Should Become Standard P...

This is not a good idea, because the blastocysts b...

2010-04-19T10:53:02.929-07:00

This is not a good idea, because the blastocysts biopsied would need to be re-vitrified (frozen again) while awaiting the CGH results. The double cryopreservation will harm the embryos.

geoff Sher

Hi Dr. Sher, Is it 'safe' to do array CGH on 5-6 ...

2010-04-14T06:33:19.167-07:00

Hi Dr. Sher,

Is it 'safe' to do array CGH on 5-6 day thawed frozen embryos? We have 11 frozen Blasts that at between 5-6 days old. We only want one more child and do elective, Single Embryos transfer (eSET). I am OK if we loose some of these 11 embryos during the testing, thawing, etc. I currently go to Reproductive Biology Associates in Atlanta, and the embryologist is excellent, and he would do the biopsy on our embryos, and then send them out for the CHG test.

I am afraid not. I do not think there are any. Ge...

2009-09-20T20:50:04.714-07:00

I am afraid not. I do not think there are any.

Geoff Sher

Hi DR Sher, Many thanks for sharing all this kno...

2009-09-20T15:57:50.568-07:00

Hi DR Sher,

Many thanks for sharing all this knowledge about IVF. It is the best information I have found so far!!

Do you know a clinic in Miami Florida that do CGH embryo selection?

Many thanks in advanced for your answer!
Anna

This is unusual unless: a) the embryo aneuploidy r...

2009-07-13T14:23:17.086-07:00

This is unusual unless: a) the embryo aneuploidy resulted from an abnormal sperm having fertilized the eggs. This is why at SIRM, we much prefer to do embryo byopsies an, b) they used array CGH (rather than metaphase GH (whicfh we use) where the diagnosis is less reliable in my opinion.

Geoff Sher

Hi Dr. Sher, I just had IVF with aCGH done in Nott...

2009-07-13T13:06:15.375-07:00

Hi Dr. Sher,
I just had IVF with aCGH done in Nottingham, UK. I went to the UK so I could do CGH with a same-cycle transfer. I was very excited about the CGH process, especially at my age of 43.
5 follicles were retrieved, all 5 contained mature eggs and all 5 fertilized. ICSI was suggested just due to the higher fertilization rates, not due to sperm issues. CGH was done on the polar bodies and on day-3 our test results showed that we had 2 chromosomally normal eggs. That's where the good news stopped though. By day-3, one of the "normal" embryos had only divided to 3-cells and the other was only at 5-cells. By day-5, the 3-cell had not changed and 5-cell was now a 6-cell. The 6-cell was transfered back (only because it was deemed "normal"), but needless to say, it did not take. My question is, why might two chromosomally normal eggs not develop properly? I really thought our chances would be higher having had 2 normal eggs.
A little history:
I'm 43- my husband is 41, no children (yet).
Both very healthy.
We have a shared HLA DQalpha, but I was given an IV intralipid to help counteract this.
2007-natural pregnancy -m/c at 5wks.
2008-4 IUIs - no pregnancy
I was on DHEA for approx 10 months prior to my trying IVF in June 2009. I can't help but wonder if that possibly had the positive, or maybe the negative, effects on my results. Did it help me produce better follicles? Did it hinder the growth of the embryos? I'm at a loss. I really thought that having 2 eggs test normal via CGH, would have resulted in at least one making it to blastocyst.
I completely admire & respect your knowledge in this area and any insight or thoughts that you may have would be greatly appreciated. I'm trying to understand what may have happened, so I can figure out what to do next. I know time is not on my side, yet we both want a baby more than anything.
Look forward to hearing from you,
Lori

Any IVF lab can order it. Might I suggest you visi...

2009-07-06T13:11:38.832-07:00

Any IVF lab can order it. Might I suggest you visit the discussion board at www.haveababy.com .

Geoff Sher

thank you, is there a lab you recommend that we us...

2009-07-06T10:41:15.993-07:00

thank you, is there a lab you recommend that we use to do this test?

I suggest that you get a Sperm DNA Integrity Assay...

2009-07-06T10:38:39.180-07:00

I suggest that you get a Sperm DNA Integrity Assay (SDIA) or a Sperm Chromatin Structure Assau (SCSA) done. They are the same tests by 2 different names. If thesae come back normal, you probably are fine.

Geoff Sher

Hi Dr. Sher, beyond a typical semen analysis, wha...

2009-07-06T09:53:53.134-07:00

Hi Dr. Sher,

beyond a typical semen analysis, what tests do you recommend to determine if sperm is competent?

we have been ttc for over a year, including two failed ivfs, our eggs are fertilized using ICSI but we have not had any blasts make it to day 5 so no transfers.

if you could list all tests you'd recommend along with where/how we can be tested i'd appreciate it.

thank you